Molecular cloning and expression of a new class of ortho-diphenol-O-methyltransferases induced in tobacco (Nicotiana tabacum L.) leaves by infection or elicitor treatment.
Identifieur interne : 002C48 ( Main/Exploration ); précédent : 002C47; suivant : 002C49Molecular cloning and expression of a new class of ortho-diphenol-O-methyltransferases induced in tobacco (Nicotiana tabacum L.) leaves by infection or elicitor treatment.
Auteurs : L. Pellegrini [France] ; P. Geoffroy ; B. Fritig ; M. LegrandSource :
- Plant physiology [ 0032-0889 ] ; 1993.
Descripteurs français
- KwdFr :
- ARN (isolement et purification), Banque de gènes (MeSH), Cartographie de restriction (MeSH), Catechol O-methyltransferase (biosynthèse), Catechol O-methyltransferase (composition chimique), Catechol O-methyltransferase (isolement et purification), Clonage moléculaire (MeSH), Données de séquences moléculaires (MeSH), Expression des gènes (MeSH), Fragments peptidiques (composition chimique), Gènes de plante (MeSH), Induction enzymatique (MeSH), Isoenzymes (biosynthèse), Isoenzymes (composition chimique), Isoenzymes (isolement et purification), Similitude de séquences d'acides aminés (MeSH), Sondes d'ADN (MeSH), Séquence d'acides aminés (MeSH), Séquence nucléotidique (MeSH), Tabac (enzymologie), Tabac (génétique), Tabac (microbiologie), Technique de Northern (MeSH), Technique de Southern (MeSH), Virus de la mosaïque du tabac (physiologie), Végétaux toxiques (MeSH).
- MESH :
- biosynthèse : Catechol O-methyltransferase, Isoenzymes.
- composition chimique : Catechol O-methyltransferase, Fragments peptidiques, Isoenzymes.
- enzymologie : Tabac.
- génétique : Tabac.
- isolement et purification : ARN, Catechol O-methyltransferase, Isoenzymes.
- microbiologie : Tabac.
- physiologie : Virus de la mosaïque du tabac.
- Banque de gènes, Cartographie de restriction, Clonage moléculaire, Données de séquences moléculaires, Expression des gènes, Gènes de plante, Induction enzymatique, Similitude de séquences d'acides aminés, Sondes d'ADN, Séquence d'acides aminés, Séquence nucléotidique, Technique de Northern, Technique de Southern, Végétaux toxiques.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Base Sequence (MeSH), Blotting, Northern (MeSH), Blotting, Southern (MeSH), Catechol O-Methyltransferase (biosynthesis), Catechol O-Methyltransferase (chemistry), Catechol O-Methyltransferase (isolation & purification), Cloning, Molecular (MeSH), DNA Probes (MeSH), Enzyme Induction (MeSH), Gene Expression (MeSH), Gene Library (MeSH), Genes, Plant (MeSH), Isoenzymes (biosynthesis), Isoenzymes (chemistry), Isoenzymes (isolation & purification), Molecular Sequence Data (MeSH), Peptide Fragments (chemistry), Plants, Toxic (MeSH), RNA (isolation & purification), Restriction Mapping (MeSH), Sequence Homology, Amino Acid (MeSH), Tobacco (enzymology), Tobacco (genetics), Tobacco (microbiology), Tobacco Mosaic Virus (physiology).
- MESH :
- chemical , biosynthesis : Catechol O-Methyltransferase, Isoenzymes.
- chemical , chemistry : Catechol O-Methyltransferase, Isoenzymes, Peptide Fragments.
- chemical , isolation & purification : Catechol O-Methyltransferase, Isoenzymes, RNA.
- enzymology : Tobacco.
- genetics : Tobacco.
- microbiology : Tobacco.
- physiology : Tobacco Mosaic Virus.
- Amino Acid Sequence, Base Sequence, Blotting, Northern, Blotting, Southern, Cloning, Molecular, DNA Probes, Enzyme Induction, Gene Expression, Gene Library, Genes, Plant, Molecular Sequence Data, Plants, Toxic, Restriction Mapping, Sequence Homology, Amino Acid.
Abstract
In tobacco (Nicotiana tabacum L. cv Samsun NN), three distinct enzymes account for ortho-diphenol-O-methyltransferase (OMT) activity. OMT I is the major enzyme of healthy leaves, whereas enzymes OMT II and III are preferentially induced during the hypersensitive reaction to tobacco mosaic virus (TMV). Using an anti-OMT III antiserum, we isolated a partial OMT III cDNA clone by immunoscreening an expression library made from mRNA of TMV-infected tobacco leaves. Using this OMT III clone as a probe, we isolated a full-length clone with a deduced amino acid sequence encompassing all of the sequences obtained by Edman degradation of both purified proteins II and III. Thus, OMT II and III of tobacco are likely to be encoded by the same genes and to arise from different posttranslational modifications. Sequence analysis showed that this OMT clone represents a new class of OMT enzymes (class II) with a low level of similarity (53-58%) to OMTs cloned previously from other dicotyledonous plants. Southern analysis indicated that a small family of class II OMT genes inherited from ancestors related to Nicotiana sylvestris and Nicotiana tomentosiformis occurs in the tobacco genome. RNA blot analysis demonstrated that class II OMT genes, unlike class I OMT genes, are not expressed at a high constitutive level in lignified tissues of tobacco. Class II OMT transcripts were found to accumulate in tobacco leaves infected with TMV or treated with megaspermin, a proteinaceous elicitor from Phytophthora megasperma, but not in leaves treated with salicylic acid, a molecule known to trigger many defense genes. In TMV-infected or elicitor-treated tissues, a marked increase in catechol-methylating activity accompanied the accumulation of class II OMT gene products.
DOI: 10.1104/pp.103.2.509
PubMed: 7518088
PubMed Central: PMC159010
Affiliations:
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Pellegrini</name><affiliation wicri:level="4"><nlm:affiliation>Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique, Université Louis Pasteur, Strasbourg, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique, Université Louis Pasteur, Strasbourg</wicri:regionArea><placeName><region type="region">Grand Est</region><region type="old region">Alsace (région administrative)</region><settlement type="city">Strasbourg</settlement></placeName><orgName type="university">Université Strasbourg 1</orgName></affiliation></author><author><name sortKey="Geoffroy, P" sort="Geoffroy, P" uniqKey="Geoffroy P" first="P" last="Geoffroy">P. Geoffroy</name></author><author><name sortKey="Fritig, B" sort="Fritig, B" uniqKey="Fritig B" first="B" last="Fritig">B. Fritig</name></author><author><name sortKey="Legrand, M" sort="Legrand, M" uniqKey="Legrand M" first="M" last="Legrand">M. 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Pellegrini</name><affiliation wicri:level="4"><nlm:affiliation>Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique, Université Louis Pasteur, Strasbourg, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique, Université Louis Pasteur, Strasbourg</wicri:regionArea><placeName><region type="region">Grand Est</region><region type="old region">Alsace (région administrative)</region><settlement type="city">Strasbourg</settlement></placeName><orgName type="university">Université Strasbourg 1</orgName></affiliation></author><author><name sortKey="Geoffroy, P" sort="Geoffroy, P" uniqKey="Geoffroy P" first="P" last="Geoffroy">P. Geoffroy</name></author><author><name sortKey="Fritig, B" sort="Fritig, B" uniqKey="Fritig B" first="B" last="Fritig">B. 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Legrand</name></author></analytic><series><title level="j">Plant physiology</title><idno type="ISSN">0032-0889</idno><imprint><date when="1993" type="published">1993</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Base Sequence (MeSH)</term><term>Blotting, Northern (MeSH)</term><term>Blotting, Southern (MeSH)</term><term>Catechol O-Methyltransferase (biosynthesis)</term><term>Catechol O-Methyltransferase (chemistry)</term><term>Catechol O-Methyltransferase (isolation & purification)</term><term>Cloning, Molecular (MeSH)</term><term>DNA Probes (MeSH)</term><term>Enzyme Induction (MeSH)</term><term>Gene Expression (MeSH)</term><term>Gene Library (MeSH)</term><term>Genes, Plant (MeSH)</term><term>Isoenzymes (biosynthesis)</term><term>Isoenzymes (chemistry)</term><term>Isoenzymes (isolation & purification)</term><term>Molecular Sequence Data (MeSH)</term><term>Peptide Fragments (chemistry)</term><term>Plants, Toxic (MeSH)</term><term>RNA (isolation & purification)</term><term>Restriction Mapping (MeSH)</term><term>Sequence Homology, Amino Acid (MeSH)</term><term>Tobacco (enzymology)</term><term>Tobacco (genetics)</term><term>Tobacco (microbiology)</term><term>Tobacco Mosaic Virus (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN (isolement et purification)</term><term>Banque de gènes (MeSH)</term><term>Cartographie de restriction (MeSH)</term><term>Catechol O-methyltransferase (biosynthèse)</term><term>Catechol O-methyltransferase (composition chimique)</term><term>Catechol O-methyltransferase (isolement et purification)</term><term>Clonage moléculaire (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Expression des gènes (MeSH)</term><term>Fragments peptidiques (composition chimique)</term><term>Gènes de plante (MeSH)</term><term>Induction enzymatique (MeSH)</term><term>Isoenzymes (biosynthèse)</term><term>Isoenzymes (composition chimique)</term><term>Isoenzymes (isolement et purification)</term><term>Similitude de séquences d'acides aminés (MeSH)</term><term>Sondes d'ADN (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>Tabac (enzymologie)</term><term>Tabac (génétique)</term><term>Tabac (microbiologie)</term><term>Technique de Northern (MeSH)</term><term>Technique de Southern (MeSH)</term><term>Virus de la mosaïque du tabac (physiologie)</term><term>Végétaux toxiques (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Catechol O-Methyltransferase</term><term>Isoenzymes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Catechol O-Methyltransferase</term><term>Isoenzymes</term><term>Peptide Fragments</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Catechol O-Methyltransferase</term><term>Isoenzymes</term><term>RNA</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Catechol O-methyltransferase</term><term>Isoenzymes</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Catechol O-methyltransferase</term><term>Fragments peptidiques</term><term>Isoenzymes</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ARN</term><term>Catechol O-methyltransferase</term><term>Isoenzymes</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus de la mosaïque du tabac</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Tobacco Mosaic Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Blotting, Northern</term><term>Blotting, Southern</term><term>Cloning, Molecular</term><term>DNA Probes</term><term>Enzyme Induction</term><term>Gene Expression</term><term>Gene Library</term><term>Genes, Plant</term><term>Molecular Sequence Data</term><term>Plants, Toxic</term><term>Restriction Mapping</term><term>Sequence Homology, Amino Acid</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Banque de gènes</term><term>Cartographie de restriction</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Expression des gènes</term><term>Gènes de plante</term><term>Induction enzymatique</term><term>Similitude de séquences d'acides aminés</term><term>Sondes d'ADN</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Technique de Northern</term><term>Technique de Southern</term><term>Végétaux toxiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In tobacco (Nicotiana tabacum L. cv Samsun NN), three distinct enzymes account for ortho-diphenol-O-methyltransferase (OMT) activity. OMT I is the major enzyme of healthy leaves, whereas enzymes OMT II and III are preferentially induced during the hypersensitive reaction to tobacco mosaic virus (TMV). Using an anti-OMT III antiserum, we isolated a partial OMT III cDNA clone by immunoscreening an expression library made from mRNA of TMV-infected tobacco leaves. Using this OMT III clone as a probe, we isolated a full-length clone with a deduced amino acid sequence encompassing all of the sequences obtained by Edman degradation of both purified proteins II and III. Thus, OMT II and III of tobacco are likely to be encoded by the same genes and to arise from different posttranslational modifications. Sequence analysis showed that this OMT clone represents a new class of OMT enzymes (class II) with a low level of similarity (53-58%) to OMTs cloned previously from other dicotyledonous plants. Southern analysis indicated that a small family of class II OMT genes inherited from ancestors related to Nicotiana sylvestris and Nicotiana tomentosiformis occurs in the tobacco genome. RNA blot analysis demonstrated that class II OMT genes, unlike class I OMT genes, are not expressed at a high constitutive level in lignified tissues of tobacco. Class II OMT transcripts were found to accumulate in tobacco leaves infected with TMV or treated with megaspermin, a proteinaceous elicitor from Phytophthora megasperma, but not in leaves treated with salicylic acid, a molecule known to trigger many defense genes. In TMV-infected or elicitor-treated tissues, a marked increase in catechol-methylating activity accompanied the accumulation of class II OMT gene products.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">7518088</PMID><DateCompleted><Year>1994</Year><Month>08</Month><Day>08</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>14</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0032-0889</ISSN><JournalIssue CitedMedium="Print"><Volume>103</Volume><Issue>2</Issue><PubDate><Year>1993</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Plant physiology</Title><ISOAbbreviation>Plant Physiol</ISOAbbreviation></Journal><ArticleTitle>Molecular cloning and expression of a new class of ortho-diphenol-O-methyltransferases induced in tobacco (Nicotiana tabacum L.) leaves by infection or elicitor treatment.</ArticleTitle><Pagination><MedlinePgn>509-17</MedlinePgn></Pagination><Abstract><AbstractText>In tobacco (Nicotiana tabacum L. cv Samsun NN), three distinct enzymes account for ortho-diphenol-O-methyltransferase (OMT) activity. OMT I is the major enzyme of healthy leaves, whereas enzymes OMT II and III are preferentially induced during the hypersensitive reaction to tobacco mosaic virus (TMV). Using an anti-OMT III antiserum, we isolated a partial OMT III cDNA clone by immunoscreening an expression library made from mRNA of TMV-infected tobacco leaves. Using this OMT III clone as a probe, we isolated a full-length clone with a deduced amino acid sequence encompassing all of the sequences obtained by Edman degradation of both purified proteins II and III. Thus, OMT II and III of tobacco are likely to be encoded by the same genes and to arise from different posttranslational modifications. Sequence analysis showed that this OMT clone represents a new class of OMT enzymes (class II) with a low level of similarity (53-58%) to OMTs cloned previously from other dicotyledonous plants. Southern analysis indicated that a small family of class II OMT genes inherited from ancestors related to Nicotiana sylvestris and Nicotiana tomentosiformis occurs in the tobacco genome. RNA blot analysis demonstrated that class II OMT genes, unlike class I OMT genes, are not expressed at a high constitutive level in lignified tissues of tobacco. Class II OMT transcripts were found to accumulate in tobacco leaves infected with TMV or treated with megaspermin, a proteinaceous elicitor from Phytophthora megasperma, but not in leaves treated with salicylic acid, a molecule known to trigger many defense genes. In TMV-infected or elicitor-treated tissues, a marked increase in catechol-methylating activity accompanied the accumulation of class II OMT gene products.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Pellegrini</LastName><ForeName>L</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique, Université Louis Pasteur, Strasbourg, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Geoffroy</LastName><ForeName>P</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Fritig</LastName><ForeName>B</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Legrand</LastName><ForeName>M</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>X71430</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Physiol</MedlineTA><NlmUniqueID>0401224</NlmUniqueID><ISSNLinking>0032-0889</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015342">DNA Probes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007527">Isoenzymes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010446">Peptide Fragments</NameOfSubstance></Chemical><Chemical><RegistryNumber>63231-63-0</RegistryNumber><NameOfSubstance UI="D012313">RNA</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.1.1.6</RegistryNumber><NameOfSubstance UI="D002394">Catechol O-Methyltransferase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015152" MajorTopicYN="N">Blotting, Northern</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015139" MajorTopicYN="N">Blotting, Southern</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002394" MajorTopicYN="N">Catechol O-Methyltransferase</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015342" MajorTopicYN="N">DNA Probes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004790" MajorTopicYN="N">Enzyme Induction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015723" MajorTopicYN="N">Gene Library</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="Y">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007527" MajorTopicYN="N">Isoenzymes</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010446" MajorTopicYN="N">Peptide Fragments</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010947" MajorTopicYN="Y">Plants, Toxic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012313" MajorTopicYN="N">RNA</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015183" MajorTopicYN="N">Restriction Mapping</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017386" MajorTopicYN="N">Sequence Homology, Amino Acid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014027" MajorTopicYN="N">Tobacco Mosaic Virus</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1993</Year><Month>10</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1993</Year><Month>10</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1993</Year><Month>10</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">7518088</ArticleId><ArticleId IdType="pii">103/2/509</ArticleId><ArticleId IdType="pmc">PMC159010</ArticleId><ArticleId IdType="doi">10.1104/pp.103.2.509</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12</Citation><ArticleIdList><ArticleId IdType="pubmed">4504350</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 1992 Jul-Aug;5(4):294-300</Citation><ArticleIdList><ArticleId IdType="pubmed">1515665</ArticleId></ArticleIdList></Reference><Reference><Citation>Immunol Lett. 1988 Feb;17(2):95-107</Citation><ArticleIdList><ArticleId IdType="pubmed">2452136</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1991 Sep;97(1):7-14</Citation><ArticleIdList><ArticleId IdType="pubmed">16668418</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Biochem Biophys. 1987 Mar;253(2):367-76</Citation><ArticleIdList><ArticleId 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Fritig</name><name sortKey="Geoffroy, P" sort="Geoffroy, P" uniqKey="Geoffroy P" first="P" last="Geoffroy">P. Geoffroy</name><name sortKey="Legrand, M" sort="Legrand, M" uniqKey="Legrand M" first="M" last="Legrand">M. Legrand</name></noCountry><country name="France"><region name="Grand Est"><name sortKey="Pellegrini, L" sort="Pellegrini, L" uniqKey="Pellegrini L" first="L" last="Pellegrini">L. Pellegrini</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhytophthoraV1/Data/Main/Exploration
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